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Antibody Product Overview
| ELK.No | ES2549 |
| Product name | hnRNP G rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | RBMX; HNRPG; RBMXP1; RNA-binding motif protein; X chromosome; Glycoprotein p43; Heterogeneous nuclear ribonucleoprotein G; hnRNP G |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 27316 |
| Human Swiss-Prot | P38159 |
| Source | Rabbit |
| Isotype | IgG |
| Target | hnRNP G |
| Fields | >>Spliceosome |
| Gene name | RBMX |
| Protein name | RNA-binding motif protein X chromosome |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 19655 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9WV02 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 302855 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q4V898 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human hnRNP G. AA range:6-55 |
| Specificity | hnRNP G Polyclonal Antibody detects endogenous levels of hnRNP G protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | This gene belongs to the RBMY gene family which includes candidate Y chromosome spermatogenesis genes. This gene, an active X chromosome homolog of the Y chromosome RBMY gene, is widely expressed whereas the RBMY gene evolved a male-specific function in spermatogenesis. Pseudogenes of this gene, found on chromosomes 1, 4, 9, 11, and 6, were likely derived by retrotransposition from the original gene. Alternatively spliced transcript variants encoding different isoforms have been identified. A snoRNA gene (SNORD61) is found in one of its introns. [provided by RefSeq, Sep 2009], |
| Function | function:RNA-binding protein which may be involved in pre-mRNA splicing.,PTM:Arg-185 is dimethylated, probably to asymmetric dimethylarginine.,PTM:Cleavage of initiator Met is partial. If Met-1 is not removed, it is acetylated. If it is removed, Val-2 is acetylated.,PTM:O-glycosylated.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subcellular location:Component of ribonucleosomes.,subunit:Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2 |
| Subcellular location | Nucleus . Component of ribonucleosomes. Localizes in numerous small granules in the nucleus. |
| Expression | Expressed strongly in oral keratinocytes, but only weakly detected in oral squamous cell carcinomas (at protein level). |
Additional Images
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Western Blot analysis of various cells using hnRNP G Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunofluorescence analysis of HepG2 cells, using hnRNP G Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HeLa cells, using hnRNP G Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES2549-100
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