Français
English
Antibody Product Overview
| ELK.No | ES2567 |
| Product name | Hrs rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | HGS; HRS; Hepatocyte growth factor-regulated tyrosine kinase substrate; Hrs; Protein pp110 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 9146 |
| Human Swiss-Prot | O14964 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Hrs |
| Fields | >>Viral life cycle - HIV-1;>>Endocytosis;>>Phagosome |
| Gene name | HGS |
| Protein name | Hepatocyte growth factor-regulated tyrosine kinase substrate |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15239 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q99LI8 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 56084 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q9JJ50 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HRS. AA range:301-350 |
| Specificity | Hrs Polyclonal Antibody detects endogenous levels of Hrs protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 80kD |
| Background | The protein encoded by this gene regulates endosomal sorting and plays a critical role in the recycling and degradation of membrane receptors. The encoded protein sorts monoubiquitinated membrane proteins into the multivesicular body, targeting these proteins for lysosome-dependent degradation. [provided by RefSeq, Dec 2010], |
| Function | domain:Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.,function:Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the |
| Subcellular location | Cytoplasm . Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Endosome, multivesicular body membrane ; Peripheral membrane protein . Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments. . |
| Expression | Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. |
Additional Images
No image
Western Blot analysis of various cells using Hrs Polyclonal Antibody diluted at 1:500
No image
Immunofluorescence analysis of HeLa cells, using HRS Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using HRS Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Western blot analysis of lysates from 293, COLO, and HUVEC cells, using HRS Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES2567-100
: 10 Produits
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package