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Antibody Product Overview
| ELK.No | ES2579 |
| Product name | HSP β2 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | HSPB2; Heat shock protein beta-2; HspB2; DMPK-binding protein; MKBP |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 3316 |
| Human Swiss-Prot | Q16082 |
| Source | Rabbit |
| Isotype | IgG |
| Target | HSP β2 |
| Fields | >>Proteoglycans in cancer |
| Gene name | HSPB2 |
| Protein name | Heat shock protein beta-2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 69253 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q99PR8 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 161476 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O35878 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSPB2. AA range:121-170 |
| Specificity | HSP β2 Polyclonal Antibody detects endogenous levels of HSP β2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 24kD |
| Background | The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The protein is expressed preferentially in the heart and skeletal muscle. This protein regulates Myotonic Dystrophy Protein Kinase, which plays an important role in maintenance of muscle structure and function. [provided by RefSeq, Dec 2012], |
| Function | similarity:Belongs to the small heat shock protein (HSP20) family.,subunit:Associates specifically with myotonic dystrophy protein kinase (DMPK).,tissue specificity:Expressed preferentially in skeletal muscle and heart but not in the lens., |
| Subcellular location | Cytoplasm . Nucleus . Localizes to nuclear foci. |
| Expression | Expressed preferentially in skeletal muscle and heart but not in the lens. |
Additional Images
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Western Blot analysis of various cells using HSP β2 Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of A549 cells, using HSPB2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HepG2 and HeLa cells, using HSPB2 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES2579-100
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