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Antibody Product Overview
| ELK.No | ES2588 |
| Product name | IARS2 rabbit pAb |
| Reactivity | Human;Mouse;Monkey |
| Applications | WB;IHC;IF;ELISA |
| Other name | IARS2; Isoleucine--tRNA ligase; mitochondrial; Isoleucyl-tRNA synthetase; IleRS |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 55699 |
| Human Swiss-Prot | Q9NSE4 |
| Source | Rabbit |
| Isotype | IgG |
| Target | IARS2 |
| Fields | >>Aminoacyl-tRNA biosynthesis |
| Gene name | IARS2 |
| Protein name | Isoleucine--tRNA ligase mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 381314 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8BIJ6 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IARS2. AA range:71-120 |
| Specificity | IARS2 Polyclonal Antibody detects endogenous levels of IARS2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 114kD |
| Background | Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family. [provided by RefSeq, Dec 2014], |
| Function | catalytic activity:ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).,similarity:Belongs to the class-I aminoacyl-tRNA synthetase family., |
| Subcellular location | Mitochondrion matrix . |
| Expression | Brain,Epithelium,Liver,Placenta,Skin, |
Additional Images
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Western Blot analysis of various cells using IARS2 Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of NIH-3T3 cells using IARS2 Polyclonal Antibody diluted at 1:2000
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Immunofluorescence analysis of HepG2 cells, using IARS2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using IARS2 Antibody. The picture on the right is blocked with the synthesized peptide.
: AO-06-ES2588-50
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