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Antibody Product Overview
| ELK.No | ES2630 |
| Product name | Integrin β3 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | ITGB3; GP3A; Integrin beta-3; Platelet membrane glycoprotein IIIa; GPIIIa; CD antigen CD61 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3690 |
| Human Swiss-Prot | P05106 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Integrin β3 |
| Fields | >>Rap1 signaling pathway;>>Phagosome;>>PI3K-Akt signaling pathway;>>Osteoclast differentiation;>>Focal adhesion;>>ECM-receptor interaction;>>Platelet activation;>>Neutrophil extracellular trap formation;>>Hematopoietic cell lineage;>>Regulation of actin cytoskeleton;>>Thyroid hormone signaling pathway;>>Human cytomegalovirus infection;>>Human papillomavirus infection;>>Herpes simplex virus 1 infection;>>Proteoglycans in cancer;>>MicroRNAs in cancer;>>Hypertrophic cardiomyopathy;>>Arrhythmogenic right ventricular cardiomyopathy;>>Dilated cardiomyopathy;>>Fluid shear stress and atherosclerosis |
| Gene name | ITGB3 |
| Protein name | Integrin beta-3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 16416 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | O54890 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Integrin beta3. AA range:739-788 |
| Specificity | Integrin β3 Polyclonal Antibody detects endogenous levels of Integrin β3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 110kD |
| Background | The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008], |
| Function | disease:Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. Its inheritance is autosomal recessive. It is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the GPIIb-IIIa complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors.,function:Int |
| Subcellular location | Cell membrane ; Single-pass type I membrane protein . Cell projection, lamellipodium membrane . Cell junction, focal adhesion . Cell junction, synapse, postsynaptic cell membrane ; Single-pass type I membrane protein . Cell junction, synapse . |
| Expression | Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis. |
Additional Images
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Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
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Western Blot analysis of various cells using Integrin β3 Polyclonal Antibody diluted at 1:500
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Western Blot analysis of NIH-3T3 cells using Integrin β3 Polyclonal Antibody diluted at 1:500
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Integrin beta3 Antibody. The picture on the right is blocked with the synthesized peptide.
: AO-06-ES2630-50
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