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Antibody Product Overview
| ELK.No | ES2763 |
| Product name | MDFI rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | MDFI; MyoD family inhibitor; Myogenic repressor I-mf |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4188 |
| Human Swiss-Prot | Q99750 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MDFI |
| Fields | |
| Gene name | MDFI |
| Protein name | MyoD family inhibitor |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 17240 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P70331 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MDFI. AA range:103-152 |
| Specificity | MDFI Polyclonal Antibody detects endogenous levels of MDFI protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 25kD |
| Background | This protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. [provided by RefSeq, Jul 2008], |
| Function | function:Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.,similarity:Belongs to the MDFI family.,subunit:The C-terminus interacts with AXIN1 and LEF1., |
| Subcellular location | Nucleus . Cytoplasm . |
| Expression | Fetal brain,Muscle, |
Additional Images
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Western Blot analysis of various cells using MDFI Polyclonal Antibody
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Western blot analysis of lysates from Jurkat cells, using MDFI Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES2763-50
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