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Antibody Product Overview
| ELK.No | ES2801 |
| Product name | MLTK rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC |
| Other name | MLTK; ZAK; HCCS4; Mitogen-activated protein kinase kinase kinase MLT; Human cervical cancer suppressor gene 4 protein; HCCS-4; Leucine zipper- and sterile alpha motif-containing kinase; MLK-like mitogen-activated protein triple kinase; Mixe |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 51776 |
| Human Swiss-Prot | Q9NYL2 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MLTK |
| Fields | >>MAPK signaling pathway |
| Gene name | MLTK |
| Protein name | Mitogen-activated protein kinase kinase kinase MLT |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 65964 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9ESL4 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MLTK. AA range:701-750 |
| Specificity | MLTK Polyclonal Antibody detects endogenous levels of MLTK protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 91kD |
| Background | This gene is a member of the MAPKKK family of signal transduction molecules and encodes a protein with an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Activated by phosphorylation by PKN1 and autophosphorylation on Thr-161 and Ser-165.,function:Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers. Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28'.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SAM (sterile alpha motif) domain.,subcellular location:Tr |
| Subcellular location | Cytoplasm . Nucleus . Translocates to the nucleus upon ultraviolet B irradiation. . |
| Expression | Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed. |
Additional Images
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Western Blot analysis of various cells using MLTK Polyclonal Antibody diluted at 1:1000
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Immunofluorescence analysis of HepG2 cells, using MLTK Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HeLa cells, using MLTK Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES2801-100
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