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Antibody Product Overview
| ELK.No | ES2848 |
| Product name | MRP-S22 rabbit pAb |
| Reactivity | Human;Monkey;Bovine;Hamster;Cow |
| Applications | WB;IHC;IF;ELISA |
| Other name | MRPS22; C3orf5; RPMS22; GK002; 28S ribosomal protein S22; mitochondrial; MRP-S22; S22mt |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 56945 |
| Human Swiss-Prot | P82650 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MRP-S22 |
| Fields | |
| Gene name | MRPS22 |
| Protein name | 28S ribosomal protein S22 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9CXW2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPS22. AA range:231-280 |
| Specificity | MRP-S22 Polyclonal Antibody detects endogenous levels of MRP-S22 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 41kD |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene |
| Function | disease:Defects in MRPS22 are the cause of combined oxidative phosphorylation deficiency type 5 (COXPD5) [MIM:611719]. COXPD5 is an antenatal mitochondrial disease. Patients show edema, cardiomyopathy, tubulopathy, and hypotonia.,subunit:Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins., |
| Subcellular location | Mitochondrion . |
| Expression | Brain,Liver cancer,Muscle,Placenta, |
Additional Images
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Western Blot analysis of various cells using MRP-S22 Polyclonal Antibody
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Western blot analysis of mouse-brain 293T lysis using MRP-S22 antibody.
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Immunohistochemical analysis of paraffin-embedded Human skeletal muscle. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of A549 cells, using MRPS22 Antibody. The picture on the right is blocked with the synthesized peptide.
: AO-06-ES2848-50
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