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Antibody Product Overview
| ELK.No | ES2849 |
| Product name | MRP-S25 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | MRPS25; RPMS25; 28S ribosomal protein S25; mitochondrial; MRP-S25; S25mt |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 64432 |
| Human Swiss-Prot | P82663 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MRP-S25 |
| Fields | |
| Gene name | MRPS25 |
| Protein name | 28S ribosomal protein S25 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 64658 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9D125 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 297459 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q4QR80 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPS25. AA range:124-173 |
| Specificity | MRP-S25 Polyclonal Antibody detects endogenous levels of MRP-S25 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 20kD |
| Background | Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016], |
| Function | similarity:Belongs to the ribosomal protein S25/L51 family.,subunit:Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins., |
| Subcellular location | Mitochondrion . |
| Expression | Lung,Spleen, |
Additional Images
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Western Blot analysis of various cells using MRP-S25 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of lysates from 293 cells, using MRPS25 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using MRPS25 antibody.
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Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES2849-50
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