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Antibody Product Overview
| ELK.No | ES2874 |
| Product name | MYH rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | MUTYH; MYH; A/G-specific adenine DNA glycosylase; MutY homolog; hMYH |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4595 |
| Human Swiss-Prot | Q9UIF7 |
| Source | Rabbit |
| Isotype | IgG |
| Target | MYH |
| Fields | >>Base excision repair |
| Gene name | MUTYH |
| Protein name | A/G-specific adenine DNA glycosylase |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 70603 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q99P21 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 170841 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q8R5G2 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MUTYH. AA range:151-200 |
| Specificity | MYH Polyclonal Antibody detects endogenous levels of MYH protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 60kD |
| Background | This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008], |
| Function | cofactor:Binds 1 4Fe-4S cluster. The cluster is not important for the catalytic activity, but which is probably involved in the proper positioning of the enzyme along the DNA strand.,disease:Defects in MUTYH are a cause of autosomal recessive colorectal adenomatous polyposis [MIM:608456].,disease:Defects in MUTYH are a cause of gastric cancer [MIM:137215].,function:Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.,similarity:Belongs to the nth/mutY family.,similarity:Contains 1 nudix hydrolase domain., |
| Subcellular location | Nucleus . Mitochondrion . |
| Expression | Kidney, |
Additional Images
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Western Blot analysis of various cells using MYH Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunofluorescence analysis of A549 cells, using MUTYH Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HeLa and HepG2 cells, using MUTYH Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human Small intestinal stromal tumor. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES2874-50
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