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Antibody Product Overview
| ELK.No | ES2938 |
| Product name | NFATc4 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | NFATC4; NFAT3; Nuclear factor of activated T-cells; cytoplasmic 4; NF-ATc4; NFATc4; T-cell transcription factor NFAT3; NF-AT3 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4776 |
| Human Swiss-Prot | Q14934 |
| Source | Rabbit |
| Isotype | IgG |
| Target | NFAT3 |
| Fields | >>cGMP-PKG signaling pathway;>>Cellular senescence;>>Wnt signaling pathway;>>Axon guidance;>>C-type lectin receptor signaling pathway;>>Oxytocin signaling pathway;>>Hepatitis B;>>Human cytomegalovirus infection;>>Human T-cell leukemia virus 1 infection;>>Kaposi sarcoma-associated herpesvirus infection;>>Human immunodeficiency virus 1 infection |
| Gene name | NFATC4 |
| Protein name | Nuclear factor of activated T-cells cytoplasmic 4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 73181 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8K120 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NFAT3. AA range:642-691 |
| Specificity | NFATc4 Polyclonal Antibody detects endogenous levels of NFATc4 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 135kD |
| Background | This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014], |
| Function | domain:Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.,function:Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.,PTM:Phosphorylated by NFATC-kinases; dephosphorylated by calcineurin. Phosphorylated on Ser-168 and Ser-170 by FRAP1, IRAK1, MAPK7 and MAPK14, on Ser-213 and Ser-217 by MAPK8 and MAPK9, and on Ser-289 and Ser-344 by RPS6KA3. Phosphorylated by GSK3B.,PTM:Ubiquitinated, leading |
| Subcellular location | Cytoplasm, cytosol . Nucleus . When hyperphosphorylated, localizes in the cytosol. When intracellular Ca(2+) levels increase, dephosphorylation by calcineurin/PPP3CA leads to translocation into the nucleus (PubMed:11997522, PubMed:18347059). MAPK7/ERK5 and MTOR regulate NFATC4 nuclear export through phosphorylation at Ser-168 and Ser-170 (PubMed:18347059). . |
| Expression | Widely expressed, with high levels in placenta, lung, kidney, testis and ovary (PubMed:18675896). Weakly expressed in spleen and thymus (PubMed:18675896). In the hippocampus, expressed in the granular layer of the dentate gyrus, in the pyramidal neurons of CA3 region, and in the hippocampal fissure (PubMed:18675896). Expressed in the heart (at protein level) (PubMed:12370307). |
Additional Images
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Western Blot analysis of various cells using NFATc4 Polyclonal Antibody diluted at 1:500
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Immunofluorescence analysis of HeLa cells, using NFAT3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using NFAT3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from 293 cells, using NFAT3 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES2938-50
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