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Antibody Product Overview
| ELK.No | ES2940 |
| Product name | NF-YA rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | NFYA; Nuclear transcription factor Y subunit alpha; CAAT box DNA-binding protein subunit A; Nuclear transcription factor Y subunit A; NF-YA |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4800 |
| Human Swiss-Prot | P23511 |
| Source | Rabbit |
| Isotype | IgG |
| Target | NF-YA |
| Fields | >>Antigen processing and presentation;>>Spinocerebellar ataxia;>>Tuberculosis |
| Gene name | NFYA |
| Protein name | Nuclear transcription factor Y subunit alpha |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 18044 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P23708 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 29508 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P18576 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NFYA. AA range:261-310 |
| Specificity | NF-YA Polyclonal Antibody detects endogenous levels of NF-YA protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 43kD |
| Background | The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq, Jul 2008], |
| Function | function:Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.,similarity:Belongs to the NFYA/HAP2 subunit family.,subunit:Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding., |
| Subcellular location | Nucleus. |
| Expression | Testis, |
Additional Images
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Immunofluorescence analysis of Hela cell. 1,NF-YA Polyclonal Antibody(red) was diluted at 1:200(4° overnight). LC3A mouse Monoclonal Antibody(5G10)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
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Western Blot analysis of various cells using NF-YA Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of lysates from HepG2, 293, and COLO205 cells, using NFYA Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human spleen. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES2940-50
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