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Antibody Product Overview
| ELK.No | ES3094 |
| Product name | p130 Cas rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | BCAR1; CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 9564 |
| Human Swiss-Prot | P56945 |
| Source | Rabbit |
| Isotype | IgG |
| Target | p130 Cas |
| Fields | >>Rap1 signaling pathway;>>Chemokine signaling pathway;>>Focal adhesion;>>Leukocyte transendothelial migration;>>Regulation of actin cytoskeleton;>>Growth hormone synthesis, secretion and action;>>Bacterial invasion of epithelial cells;>>Shigellosis;>>Yersinia infection;>>Human cytomegalovirus infection |
| Gene name | BCAR1 |
| Protein name | Breast cancer anti-estrogen resistance protein 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12927 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q61140 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25414 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q63767 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p130 Cas. AA range:376-425 |
| Specificity | p130 Cas Polyclonal Antibody detects endogenous levels of p130 Cas protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 130kD |
| Background | BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009], |
| Function | domain:A serine-rich region promotes activation of the serum response element (SRE).,domain:Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with CASL.,domain:The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of focal adhesion kinase 1.,function:Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.,PTM:Focal adhesion kinase 1 phosphoryl |
| Subcellular location | Cell junction, focal adhesion . Cytoplasm . Cell projection, axon . Unphosphorylated form localizes in the cytoplasm (By similarity). Localizes to focal adhesion sites following integrin engagement (By similarity). . |
| Expression | Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line. |
Additional Images
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Western Blot analysis of various cells using p130 Cas Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using p130 Cas Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HUVEC cells, using p130 Cas Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES3094-100
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