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Antibody Product Overview
| ELK.No | ES3193 |
| Product name | PHKG1 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC |
| Other name | PHKG1; PHKG; Phosphorylase b kinase gamma catalytic chain; skeletal muscle isoform; Phosphorylase kinase subunit gamma-1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5260 |
| Human Swiss-Prot | Q16816 |
| Source | Rabbit |
| Isotype | IgG |
| Target | PHKG1 |
| Fields | >>Calcium signaling pathway;>>Insulin signaling pathway;>>Glucagon signaling pathway |
| Gene name | PHKG1 |
| Protein name | Phosphorylase b kinase gamma catalytic chain skeletal muscle isoform |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P07934 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human PHKG1. AA range:241-290 |
| Specificity | PHKG1 Polyclonal Antibody detects endogenous levels of PHKG1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 45kD |
| Background | This gene is a member of the Ser/Thr protein kinase family and encodes a protein with one protein kinase domain and two calmodulin-binding domains. This protein is the catalytic member of a 16 subunit protein kinase complex which contains equimolar ratios of 4 subunit types. The complex is a crucial glycogenolytic regulatory enzyme. This gene has two pseudogenes at chromosome 7q11.21 and one at chromosome 11p11.12. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012], |
| Function | catalytic activity:2 ATP + phosphorylase b = 2 ADP + phosphorylase a.,domain:The two calmodulin-binding domains appear to act in concert to bind a single molecule of calmodulin and are pseudosubstrate/autoinhibitory domains.,function:Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.,similarity:Contains 1 protein kinase domain.,subunit:Polymer of 16 chains, four each of alpha, beta, gamma, and delta. Alpha and beta are regulatory chains, gamma is the catalytic chain, and delta is calmodulin., |
| Subcellular location | cytosol,phosphorylase kinase complex, |
| Expression | PCR rescued clones,Salivary gland,Skeletal muscle, |
Additional Images
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Western Blot analysis of various cells using PHKG1 Polyclonal Antibody
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Western Blot analysis of HepG2 cells using PHKG1 Polyclonal Antibody
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Western blot analysis of the lysates from HUVECcells using PHKG1 antibody.
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES3193-50
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