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Antibody Product Overview
| ELK.No | ES3202 |
| Product name | PI-9 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | SERPINB9; PI9; Serpin B9; Cytoplasmic antiproteinase 3; CAP-3; CAP3; Peptidase inhibitor 9; PI-9 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5272 |
| Human Swiss-Prot | P50453 |
| Source | Rabbit |
| Isotype | IgG |
| Target | PI-9 |
| Fields | >>Amoebiasis |
| Gene name | SERPINB9 |
| Protein name | Serpin B9 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SERPINB9. AA range:241-290 |
| Specificity | PI-9 Polyclonal Antibody detects endogenous levels of PI-9 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 40kD |
| Background | This gene encodes a member of the serine protease inhibitor family which are also known as serpins. The encoded protein belongs to a subfamily of intracellular serpins. This protein inhibits the activity of the effector molecule granzyme B. Overexpression of this protein may prevent cytotoxic T-lymphocytes from eliminating certain tumor cells. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Mar 2012], |
| Function | function:Granzyme B inhibitor.,similarity:Belongs to the serpin family.,similarity:Belongs to the serpin family. Ov-serpin subfamily., |
| Subcellular location | Cytoplasm. |
| Expression | Bone marrow,Human esophagus tumor,Placenta,Testis, |
Additional Images
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Immunofluorescence analysis of A549. 1,primary Antibody was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 488 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
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Western Blot analysis of various cells using PI-9 Polyclonal Antibody diluted at 1:1000
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using SERPINB9 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COLO, HUVEC, and Jurkat cells, using SERPINB9 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES3202-50
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