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Antibody Product Overview
| ELK.No | ES3291 |
| Product name | Rab 35 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB; ELISA;IHC |
| Other name | RAB35; RAB1C; RAY; Ras-related protein Rab-35; GTP-binding protein RAY; Ras-related protein Rab-1C |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 11021 |
| Human Swiss-Prot | Q15286 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rab 35 |
| Fields | >>Endocytosis |
| Gene name | RAB35 |
| Protein name | Ras-related protein Rab-35 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 77407 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q6PHN9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 288700 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q5U316 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RAB35. AA range:99-148 |
| Specificity | Rab 35 Polyclonal Antibody detects endogenous levels of Rab 35 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 24kD |
| Background | function:Possesses GTPase activity.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV., |
| Function | function:Possesses GTPase activity.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV., |
| Subcellular location | Cell membrane ; Lipid-anchor ; Cytoplasmic side . Membrane, clathrin-coated pit . Cytoplasmic vesicle, clathrin-coated vesicle . Endosome . Melanosome . Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065). . |
| Expression | Brain,Muscle,Uterus, |
Additional Images
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Western Blot analysis of various cells using Rab 35 Polyclonal Antibody
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Western blot analysis of RAB35 Antibody. The lane on the right is blocked with the RAB35 peptide.
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Western blot analysis of the lysates from HT-29 cells using RAB35 antibody.
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Immunohistochemical analysis of paraffin-embedded human lung cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES3291-50
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