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Antibody Product Overview
| ELK.No | ES3296 |
| Product name | Rabaptin-5β rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | RABEP2; RABPT5B; Rab GTPase-binding effector protein 2; Rabaptin-5beta |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 79874 |
| Human Swiss-Prot | Q9H5N1 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rabaptin-5β |
| Fields | |
| Gene name | RABEP2 |
| Protein name | Rab GTPase-binding effector protein 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 70314 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q91WG2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 80754 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q62835 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RABEP2. AA range:353-402 |
| Specificity | Rabaptin-5β Polyclonal Antibody detects endogenous levels of Rabaptin-5β protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 64kD |
| Background | function:Plays a role in membrane trafficking and in homotypic early endosome fusion.,similarity:Belongs to the rabaptin family.,subunit:Heterodimer with RABGEF1. The dimer binds RAB5A that has been activated by GTP-binding., |
| Function | function:Plays a role in membrane trafficking and in homotypic early endosome fusion.,similarity:Belongs to the rabaptin family.,subunit:Heterodimer with RABGEF1. The dimer binds RAB5A that has been activated by GTP-binding., |
| Subcellular location | Cytoplasm . Early endosome . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, cilium basal body . |
| Expression | Hepatoma,Mammary gland,Testis, |
Additional Images
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Western Blot analysis of various cells using Rabaptin-5β Polyclonal Antibody
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from HeLa, Jurkat, 293, and HUVEC cells, using RABEP2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using RABEP2 antibody.
: AO-06-ES3296-100
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