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Antibody Product Overview
| ELK.No | ES3297 |
| Product name | Rabenosyn-5 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA;IHC |
| Other name | ZFYVE20; Rabenosyn-5; 110 kDa protein; FYVE finger-containing Rab5 effector protein rabenosyn-5; Zinc finger FYVE domain-containing protein 20 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 64145 |
| Human Swiss-Prot | Q9H1K0 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rabenosyn-5 |
| Fields | >>Endocytosis |
| Gene name | ZFYVE20 |
| Protein name | Rabenosyn-5 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 78287 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q80Y56 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ZFYVE20. AA range:145-194 |
| Specificity | Rabenosyn-5 Polyclonal Antibody detects endogenous levels of Rabenosyn-5 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 89kD |
| Background | This gene encodes a protein that belongs to the FYVE zinc finger family of proteins. The encoded protein interacts with Ras-related proteins that regulate membrane trafficking. A missense mutation in this gene is associated with a defect in the early endocytic pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014], |
| Function | function:Rab4/Rab5 effector protein acting in early endocytic membrane fusion and membrane trafficking of recycling endosomes. Required for endosome fusion either homotypically or with clathrin coated vesicles. Plays a role in the lysosomal trafficking of cathepsin D from the Golgi to lysosomes. Also promotes the recycling of transferrin directly from early endosomes to the plasma membrane. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate (PtdInsP3).,similarity:Contains 1 C2H2-type zinc finger.,similarity:Contains 1 FYVE-type zinc finger.,similarity:Contains 1 UIM (ubiquitin-interacting motif) repeat.,subunit:Interacts with EHD1, RAB4A, RAB5A, RAB22A, RAB24 and VPS45. Binds simultaneously to RAB4A and RAB5A in vitro. Interacts with RAB4A and RAB5A that has been activated by GTP binding., |
| Subcellular location | Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane ; Lipid-anchor. Enriched in endosomes that are in close proximity to clathrin-enriched regions at the cell surface. . |
| Expression | Brain,Cervix carcinoma,Epithelium,Human salivary gland, |
Additional Images
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Western Blot analysis of various cells using Rabenosyn-5 Polyclonal Antibody diluted at 1:1000
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Western blot analysis of lysates from HUVEC and Jurkat cells, using ZFYVE20 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from COLO205 cells using ZFYVE20 antibody.
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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES3297-100
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