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Antibody Product Overview
| ELK.No | ES3321 |
| Product name | Rak rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | FRK; PTK5; RAK; Tyrosine-protein kinase FRK; FYN-related kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 2444 |
| Human Swiss-Prot | P42685 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rak |
| Fields | |
| Gene name | FRK |
| Protein name | Tyrosine-protein kinase FRK |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 14302 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q922K9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 79209 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q62662 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human FRK. AA range:71-120 |
| Specificity | Rak Polyclonal Antibody detects endogenous levels of Rak protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 55kD |
| Background | The protein encoded by this gene belongs to the TYR family of protein kinases. This tyrosine kinase is a nuclear protein and may function during G1 and S phase of the cell cycle and suppress growth. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SH2 domain.,similarity:Contains 1 SH3 domain.,tissue specificity:Restricted to cells lines derived from tissues of lymphoid, brain, breast, colon and bladder origin., |
| Subcellular location | Cytoplasm . Nucleus . Predominantly found in the nucleus, with a small fraction found in the cell periphery. |
| Expression | Predominantly expressed in epithelial derived cell lines and tissues, especially normal liver, kidney, breast and colon. |
Additional Images
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Western Blot analysis of various cells using Rak Polyclonal Antibody
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Immunofluorescence analysis of HUVEC cells, using FRK Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FRK Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, using FRK Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES3321-100
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