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Antibody Product Overview
| ELK.No | ES3355 |
| Product name | RhoGEF p115 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA;IHC |
| Other name | ARHGEF1; Rho guanine nucleotide exchange factor 1; 115 kDa guanine nucleotide exchange factor; p115-RhoGEF; p115RhoGEF; Sub1.5 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 9138 |
| Human Swiss-Prot | Q92888 |
| Source | Rabbit |
| Isotype | IgG |
| Target | RhoGEF p115 |
| Fields | >>Vascular smooth muscle contraction;>>Platelet activation;>>Regulation of actin cytoskeleton;>>Parathyroid hormone synthesis, secretion and action;>>Pathogenic Escherichia coli infection;>>Yersinia infection;>>Human cytomegalovirus infection;>>Pathways in cancer;>>Proteoglycans in cancer;>>Lipid and atherosclerosis |
| Gene name | ARHGEF1 |
| Protein name | Rho guanine nucleotide exchange factor 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 16801 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q61210 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | Q9Z1I6 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ARHGEF1. AA range:162-211 |
| Specificity | RhoGEF p115 Polyclonal Antibody detects endogenous levels of RhoGEF p115 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 105kD |
| Background | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008], |
| Function | domain:The DH domain is involved in interaction with CCPG1.,domain:The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.,function:Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12.,PTM:Phosphorylated by PKCA.,sequence caution:Contaminating sequence. Sequence of unknown origin in the N-terminal part.,similarity:Contains 1 DH (DBL-homology) domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 RGSL (RGS-like) domain.,subcellular location:Translocated to the membrane by activ |
| Subcellular location | Cytoplasm . Membrane . Translocated to the membrane by activated GNA13 or LPA stimulation. |
| Expression | Ubiquitously expressed. |
Additional Images
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Western Blot analysis of various cells using RhoGEF p115 Polyclonal Antibody
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Western blot analysis of the lysates from HepG2 cells using ARHGEF1 antibody.
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Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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: AO-06-ES3355-50
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