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Antibody Product Overview
| ELK.No | ES3406 |
| Product name | SAP 14 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | SF3B14; CGI-110; HSPC175; HT006; Pre-mRNA branch site protein p14; SF3b 14 kDa subunit |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 51639 |
| Human Swiss-Prot | Q9Y3B4 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SAP 14 |
| Fields | >>Spliceosome |
| Gene name | SF3B14 |
| Protein name | Pre-mRNA branch site protein p14 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 66055 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P59708 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SF3B14. AA range:76-125 |
| Specificity | SAP 14 Polyclonal Antibody detects endogenous levels of SAP 14 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 14kD |
| Background | This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008], |
| Function | function:Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.,similarity:Contains 1 RRM (RNA recognition motif) domain.,subunit:Interacts with SF3B1/SF3b155 in the region of amino acids 255-424. Interacts to a lesser extent with SF3b130., |
| Subcellular location | Nucleus . |
| Expression | Hypothalamus,Testis,Umbilical cord blood,Uterus, |
Additional Images
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Western Blot analysis of various cells using SAP 14 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of lysates from HepG2 cells, using SF3B14 Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES3406-50
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