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Antibody Product Overview
| ELK.No | ES3407 |
| Product name | SAPAP1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | DLGAP1; DAP1; GKAP; Disks large-associated protein 1; DAP-1; Guanylate kinase-associated protein; hGKAP; PSD-95/SAP90-binding protein 1; SAP90/PSD-95-associated protein 1; SAPAP1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 9229 |
| Human Swiss-Prot | O14490 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SAPAP1 |
| Fields | >>Glutamatergic synapse |
| Gene name | DLGAP1 |
| Protein name | Disks large-associated protein 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 224997 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9D415 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 65040 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P97836 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human DLGP1. AA range:791-840 |
| Specificity | SAPAP1 Polyclonal Antibody detects endogenous levels of SAPAP1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 108kD |
| Background | function:Part of the postsynaptic scaffold in neuronal cells.,similarity:Belongs to the SAPAP family.,subcellular location:Found in postsynaptic density of neuronal cells.,subunit:Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.,tissue specificity:Expressed in brain., |
| Function | function:Part of the postsynaptic scaffold in neuronal cells.,similarity:Belongs to the SAPAP family.,subcellular location:Found in postsynaptic density of neuronal cells.,subunit:Interacts with guanylate kinase-like domain of DLG1, DLG2, DLG3, DLG4 and AIP1. Interacts with the PDZ domain of SHANK1, SHANK2 and SHANK3. Found in a complex with DLG4 and SHANK1, SHANK2 or SHANK3. Found in a complex with DLG4 and BEGAIN. Interacts with DYL2 and LRFN1.,tissue specificity:Expressed in brain., |
| Subcellular location | Cell membrane ; Peripheral membrane protein . Cell junction, synapse, postsynaptic density . Cell junction, synapse . |
| Expression | Expressed in brain. |
Additional Images
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Western Blot analysis of various cells using SAPAP1 Polyclonal Antibody
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Immunofluorescence analysis of HeLa cells, using DLGP1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HepG2 cells, using DLGP1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES3407-50
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