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Antibody Product Overview
| ELK.No | ES3484 |
| Product name | Spi-C rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA;IHC |
| Other name | SPIC; Transcription factor Spi-C |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 121599 |
| Human Swiss-Prot | Q8N5J4 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Spi-C |
| Fields | |
| Gene name | SPIC |
| Protein name | Transcription factor Spi-C |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q6P3D7 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SPIC. AA range:131-180 |
| Specificity | Spi-C Polyclonal Antibody detects endogenous levels of Spi-C protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000 |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 29kD |
| Background | The protein encoded by this gene regulates the development of red pulp macrophages, which are necessary for iron homeostasis and the recycling of red blood cells. [provided by RefSeq, Aug 2016], |
| Function | function:Probable transcription factor that binds to the PU-box, a purine-rich DNA sequence (5'-GAGGA[AT]-3') that can act as a lymphoid-specific enhancer.,similarity:Belongs to the ETS family.,similarity:Contains 1 ETS DNA-binding domain.,subunit:Binds DNA as a monomer.,tissue specificity:Preferentially detected in fetal and adult spleen, lymph nodes and at lower levels in bone marrow and fetal liver., |
| Subcellular location | Nucleus . |
| Expression | Preferentially detected in fetal and adult spleen, lymph nodes and at lower levels in bone marrow and fetal liver. |
Additional Images
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Western Blot analysis of various cells using Spi-C Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Western blot analysis of lysates from HepG2 and HeLa cells, using SPIC Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from Jurkat cells using SPIC antibody.
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Immunohistochemical analysis of paraffin-embedded human oophoroma. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
: AO-06-ES3484-100
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