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Antibody Product Overview
| ELK.No | ES3494 |
| Product name | SR-4 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | HTR4; 5-hydroxytryptamine receptor 4; 5-HT-4; 5-HT4; Serotonin receptor 4 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3360 |
| Human Swiss-Prot | Q13639 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SR-4 |
| Fields | >>Calcium signaling pathway;>>cAMP signaling pathway;>>Neuroactive ligand-receptor interaction;>>Serotonergic synapse |
| Gene name | HTR4 |
| Protein name | 5-hydroxytryptamine receptor 4 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 15562 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P97288 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 25324 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q62758 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human 5-HT-4. AA range:21-70 |
| Specificity | SR-4 Polyclonal Antibody detects endogenous levels of SR-4 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 43kD |
| Background | This gene is a member of the family of serotonin receptors, which are G protein coupled receptors that stimulate cAMP production in response to serotonin (5-hydroxytryptamine). The gene product is a glycosylated transmembrane protein that functions in both the peripheral and central nervous system to modulate the release of various neurotransmitters. Multiple transcript variants encoding proteins with distinct C-terminal sequences have been described. [provided by RefSeq, May 2010], |
| Function | alternative products:Additional isoforms seem to exist,function:This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.,similarity:Belongs to the G-protein coupled receptor 1 family.,subcellular location:Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.,subunit:Isoform 5-HT4(A) interacts with MAGI2, MPP3, SLC9A3R1 and SNX27 isoforms 1 and 2. Isoform 5-HT4(E) interacts with INADL, NOS1 and SEC23A. Isoform 5-HT4(A) forms a complex including SLC9A3R1 and EZR.,tissue specificity:Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle. |
| Subcellular location | Cell membrane; Multi-pass membrane protein. Endosome. Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli. . |
| Expression | Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle. |
Additional Images
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Western Blot analysis of various cells using SR-4 Polyclonal Antibody
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Immunofluorescence analysis of HeLa cells, using 5-HT-4 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat/COLO205, using 5-HT-4 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from 293 cells using Collagen I α2 antibody.
: AO-06-ES3494-50
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