Français
English
Antibody Product Overview
| ELK.No | ES3588 |
| Product name | TFIIIB90-1 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | BRF1; BRF; GTF3B; TAF3B2; TAF3C; Transcription factor IIIB 90 kDa subunit; TFIIIB90; hTFIIIB90; B-related factor 1; BRF-1; hBRF; TAF3B2; TATA box-binding protein-associated factor; RNA polymerase III, subunit 2 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 2972 |
| Human Swiss-Prot | Q92994 |
| Source | Rabbit |
| Isotype | IgG |
| Target | TFIIIB90-1 |
| Fields | |
| Gene name | BRF1 |
| Protein name | Transcription factor IIIB 90 kDa subunit |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q8CFK2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TF3B. AA range:231-280 |
| Specificity | TFIIIB90-1 Polyclonal Antibody detects endogenous levels of TFIIIB90-1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 74kD |
| Background | This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011], |
| Function | function:General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the TFIIB family.,similarity:Contains 1 TFIIB-type zinc finger.,subunit:TFIIIB comprises at least the TATA-binding protein (TBP) and the B-related factor 1 (BRF1/TFIIIB90). Interacts with BDP1., |
| Subcellular location | Nucleus . |
| Expression | Brain,Epithelium,PCR rescued clones |
Additional Images
No image
Western Blot analysis of various cells using TFIIIB90-1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
No image
Immunofluorescence analysis of MCF7 cells, using TF3B Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TF3B Antibody. The picture on the right is blocked with the synthesized peptide.
No image
Western blot analysis of lysates from HeLa and Jurkat cells, using TF3B Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES3588-50
: 10 Produits
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package