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Antibody Product Overview
| ELK.No | ES3719 |
| Product name | YAP rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | YAP1; YAP65; Yorkie homolog; 65 kDa Yes-associated protein; YAP65 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 10413 |
| Human Swiss-Prot | P46937 |
| Source | Rabbit |
| Isotype | IgG |
| Target | YAP |
| Fields | >>Hippo signaling pathway;>>Hippo signaling pathway - multiple species |
| Gene name | YAP1 |
| Protein name | Yorkie homolog |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 22601 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P46938 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 363014 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q2EJA0 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human YAP. AA range:281-330 |
| Specificity | YAP Polyclonal Antibody detects endogenous levels of YAP protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 67kD |
| Background | This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013], |
| Function | PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 WW domain.,subunit:Binds to the SH3 domain of the YES kinase. Binds to WBP1 and WBP2. Binds, in vitro, through the WW1 domain, to neural isoforms of ENAH that contain the PPSY motif., |
| Subcellular location | Cytoplasm . Nucleus . Both phosphorylation and cell density can regulate its subcellular localization (PubMed:18158288, PubMed:20048001). Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus (PubMed:18158288, PubMed:20048001). At low density, predominantly nuclear and is translocated to the cytoplasm at high density (PubMed:18158288, PubMed:20048001, PubMed:25849865). PTPN14 induces translocation from the nucleus to the cytoplasm (PubMed:22525271). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm at the blastocyst and epiblast stages (By similarity). . |
| Expression | Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). |
Additional Images
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Western blot analysis of lysates from 1)Hela cell , 2)Hela cells knockdown by siRNA (F:GGUCAGAGAUACUUCUUAATT,R:UUAAGAAGUAUCUCUGACCTT), (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(Immunoway:RS23920)was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody(5B7) (Immunoway:YM3029) antibody was diluted at 1:5000 as loading control, 4° over night, Dylight 680 secondary antibody(Immunoway:RS23710)was diluted at 1:10000, 37° 1hour.
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Immunofluorescence analysis of Hela cell. 1,YAP Polyclonal Antibody(red) was diluted at 1:200(4° overnight). GAPDH Monoclonal Antibody(2B8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
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Immunofluorescence analysis of rat-spleen tissue. 1,YAP Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunofluorescence analysis of rat-spleen tissue. 1,YAP Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
: AO-06-ES3719-100
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