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Antibody Product Overview
| ELK.No | ES3761 |
| Product name | ENT1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;ELISA |
| Other name | SLC29A1; ENT1; Equilibrative nucleoside transporter 1; Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter;Equilibrative NBMPR-sensitive nucleoside transporter; Nucleoside transporter, es-type; Solute carrier family 29 member 1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 2030 |
| Human Swiss-Prot | Q99808 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ENT1 |
| Fields | >>Alcoholism |
| Gene name | SLC29A1 |
| Protein name | Equilibrative nucleoside transporter 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 63959 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9JIM1 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 63997 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O54698 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ENT1. AA range:15-64 |
| Specificity | ENT1 Polyclonal Antibody detects endogenous levels of ENT1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 55kD |
| Background | This gene is a member of the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008], |
| Function | function:Mediates both influx and efflux of nucleosides across the membrane (equilibrative transporter). It is sensitive (ES) to low concentrations of the inhibitor nitrobenzylmercaptopurine riboside (NBMPR) and is sodium-independent. It has a higher affinity for adenosine. Inhibited by dipyridamole and dilazep (anticancer chemotherapeutics drugs).,PTM:Glycosylated.,similarity:Belongs to the SLC29A transporter family.,tissue specificity:Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen., |
| Subcellular location | Basolateral cell membrane; Multi-pass membrane protein. Apical cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Predominantly localized in the basolateral membrane in polarized MDCK cells. |
| Expression | Detected in erythrocytes (at protein level). Expressed in heart, brain, mammary gland, erythrocytes and placenta, and also in fetal liver and spleen. |
Additional Images
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Western Blot analysis of extracts from Jurkat, K562, NIH-3T3 cells, using ENT1 Polyclonal Antibody. Antibody was diluted at 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Western blot analysis of lysates from MDA-MB-435 cells, using ENT1 antibody.
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: AO-06-ES3761-50
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