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Antibody Product Overview
| ELK.No | ES3795 |
| Product name | DNA pol ε B rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | POLE2; DPE2; DNA polymerase epsilon subunit 2; DNA polymerase II subunit 2; DNA polymerase epsilon subunit B |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 5427 |
| Human Swiss-Prot | P56282 |
| Source | Rabbit |
| Isotype | IgG |
| Target | DNA pol ε B |
| Fields | >>DNA replication;>>Base excision repair;>>Nucleotide excision repair |
| Gene name | POLE2 |
| Protein name | DNA polymerase epsilon subunit 2 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | O54956 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from the Internal region of human DNA pol ε B. |
| Specificity | DNA pol ε B Polyclonal Antibody detects endogenous levels of DNA pol ε B protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 59kD |
| Background | catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Participates in DNA repair and in chromosomal DNA replication.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,similarity:Belongs to the DNA polymerase epsilon subunit B family.,subunit:Consists of four subunits: POLE, POLE2, POLE3 and POLE4., |
| Function | catalytic activity:Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).,function:Participates in DNA repair and in chromosomal DNA replication.,miscellaneous:In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.,similarity:Belongs to the DNA polymerase epsilon subunit B family.,subunit:Consists of four subunits: POLE, POLE2, POLE3 and POLE4., |
| Subcellular location | Nucleus. |
| Expression | T cells, |
Additional Images
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Western Blot analysis of extracts from Jurkat cells, using DNA pol ε B Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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: AO-06-ES3795-50
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