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Antibody Product Overview
| ELK.No | ES3811 |
| Product name | UBE2A/B rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | UBE2A; RAD6A; Ubiquitin-conjugating enzyme E2 A; RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A; UBE2B; RAD6B; Ubiquitin-conjugating enzyme E2 B;RAD6 homolog B; HR6B; hHR6B; Ubiquitin carrier protein B; Ubiquitin-conjugating enzyme E2-17 kDa; Ubiquitin-protein ligase B |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 7320 |
| Human Swiss-Prot | P49459/P63146 |
| Source | Rabbit |
| Isotype | IgG |
| Target | UBE2A/B |
| Fields | >>Ubiquitin mediated proteolysis |
| Gene name | UBE2A/UBE2B |
| Protein name | Ubiquitin-conjugating enzyme E2 A/Ubiquitin-conjugating enzyme E2 B |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | |
| Mouse Swiss link | |
| Rat gene ID | 81816 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P63149 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from the C-terminal region of human UBE2A/B. |
| Specificity | UBE2A/B Polyclonal Antibody detects endogenous levels of UBE2A/B protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 17kD |
| Background | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013], |
| Function | catalytic activity:ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.,caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,function:Catalyzes the covalent attachment of ubiquitin to other proteins. Required for postreplication repair of UV-damaged DNA.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subunit:Interacts with RAD18., |
| Subcellular location | chromatin,nuclear chromatin,XY body,cytoplasm,cytosol,HULC complex, |
| Expression | Lung,Skin,Subthalamic nucleus,Thyroid, |
Additional Images
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Western Blot analysis of extracts from Jurkat cells, using UBE2A/B Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
: AO-06-ES3811-50
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