Français
English
Antibody Product Overview
| ELK.No | ES3868 |
| Product name | IL-10 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | IL10; Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 3586 |
| Human Swiss-Prot | P22301 |
| Source | Rabbit |
| Isotype | IgG |
| Target | IL-10 |
| Fields | >>Cytokine-cytokine receptor interaction;>>Viral protein interaction with cytokine and cytokine receptor;>>FoxO signaling pathway;>>C-type lectin receptor signaling pathway;>>JAK-STAT signaling pathway;>>T cell receptor signaling pathway;>>Intestinal immune network for IgA production;>>Pertussis;>>Yersinia infection;>>Leishmaniasis;>>Chagas disease;>>African trypanosomiasis;>>Malaria;>>Toxoplasmosis;>>Amoebiasis;>>Staphylococcus aureus infection;>>Tuberculosis;>>Asthma;>>Autoimmune thyroid disease;>>Inflammatory bowel disease;>>Systemic lupus erythematosus;>>Allograft rejection |
| Gene name | IL10 |
| Protein name | Interleukin-10 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P18893 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human IL10. AA range:71-120 |
| Specificity | IL-10 Polyclonal Antibody detects endogenous levels of IL-10 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 20kD |
| Background | The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011], |
| Function | disease:Defects in IL10 are a cause of susceptibility to Crohn disease (CD) [MIM:266600]. CD is a form of remitting inflammatory bowel disease (IBD). CD may involve any part of the gastrointestinal tract, but most frequently the terminal ileum and colon. Bowel inflammation is transmural and discontinuous. Crohn disease is commonly classified as autoimmune disease.,function:Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.,online information:Interleukin-10 entry,online information:The Singapore human mutation and polymorphism database,similarity:Belongs to the IL-10 family.,subunit:Homodimer.,tissue specificity:Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types., |
| Subcellular location | Secreted. |
| Expression | Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. |
Additional Images
No image
Western Blot analysis of HepG2, Mouse liver cells using IL-10 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
No image
Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
No image
Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:100
No image
Immunohistochemical analysis of paraffin-embedded Human Cerebral cortex. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
: AO-06-ES3868-100
: 10 Produits
Hurry! only 10 items left in stock.
Use collapsible tabs for more detailed information that will help customers make a purchasing decision.
Ex: Shipping and return policies, size guides, and other common questions.
- Paste the label on a flat surface on the package
- Make sure that both 1D and 2D barcodes are clearly visible
- Ensure that the label is smooth and isn’t creased or wrinkled
- Check for any tears, dents, holes or scratches
- Pack your product tightly, with the right size packaging
- Ensure both barcodes are on a flat surface of the package