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Antibody Product Overview
| ELK.No | ES3870 |
| Product name | AFP rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | AFP; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 174 |
| Human Swiss-Prot | P02771 |
| Source | Rabbit |
| Isotype | IgG |
| Target | AFP |
| Fields | >>Hippo signaling pathway |
| Gene name | AFP |
| Protein name | Alpha-fetoprotein |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | P02772 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human AFP. AA range:371-420 |
| Specificity | AFP Polyclonal Antibody detects endogenous levels of AFP protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 70kD |
| Background | This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008], |
| Function | developmental stage:Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.,function:Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.,online information:Alpha-fetoprotein entry,PTM:Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.,PTM:Sulfated.,similarity:Belongs to the ALB/AFP/VDB family.,similarity:Contains 3 albumin domains.,subunit:Dimeric and trimeric forms have been found in addition to the monomeric form.,tissue |
| Subcellular location | Secreted. |
| Expression | Plasma. Synthesized by the fetal liver and yolk sac. |
Additional Images
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Western Blot analysis of mouse liver, mouse serum cells using AFP Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded rat-brain, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded mouse-brain, antibody was diluted at 1:100
: AO-06-ES3870-100
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