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Antibody Product Overview
| ELK.No | ES3886 |
| Product name | c-FLIP rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | CFLAR; CASH; CASP8AP1; CLARP; MRIT; CASP8 and FADD-like apoptosis regulator; Caspase homolog; CASH; Caspase-eight-related protein; Casper; Caspase-like apoptosis regulatory protein;CLARP; Cellular FLICE-like inhibitory protein; c-FLIP; FADD-like antiapoptotic molecule 1; FLAME-1; Inhibitor of FLICE; I-FLICE; MACH-related inducer of toxicity; MRIT; Usurpin |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 8837 |
| Human Swiss-Prot | O15519 |
| Source | Rabbit |
| Isotype | IgG |
| Target | c-FLIP |
| Fields | >>NF-kappa B signaling pathway;>>Autophagy - animal;>>Apoptosis;>>Necroptosis;>>TNF signaling pathway;>>Chagas disease;>>Hepatitis C |
| Gene name | CFLAR |
| Protein name | CASP8 and FADD-like apoptosis regulator |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | O35732 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human CFLAR. AA range:1-50 |
| Specificity | c-FLIP Polyclonal Antibody detects endogenous levels of c-FLIP protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 55kD |
| Background | The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists. [provided by RefSeq, Feb 2011], |
| Function | domain:The caspase domain lacks the active sites residues involved in catalysis.,function:Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.,induction:Repressed by IL-2 after TCR stimulation, during progression to the S-phase of the cell cycle.,PTM:Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.,similarity:Belongs to the peptidase C14A family.,similarity:Contains |
| Subcellular location | cytoplasm,cytosol,death-inducing signaling complex,CD95 death-inducing signaling complex,membrane raft,ripoptosome, |
| Expression | Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle. |
Additional Images
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Western Blot analysis of SW480, SKOV3, HepG2 cells using c-FLIP Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Western blot analysis of lysate from SW480 cells, using CFLAR Antibody.
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: AO-06-ES3886-50
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