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Antibody Product Overview
| ELK.No | ES3894 |
| Product name | Amylase rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | AMY1A; AMY1; AMY1B; AMY1; AMY1C; AMY1; Alpha-amylase 1; 1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; AMY2A; Pancreatic alpha-amylase; PA;1,4-alpha-D-glucan glucanohydrolase; AMY2B; Alpha-amylase 2B; 1,4-alpha-D-glucan glucanohydrolase 2B; Carcinoid alpha-amylase |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 276 |
| Human Swiss-Prot | P04745 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Amylase |
| Fields | |
| Gene name | AMY1A/AMY1B/AMY1C/AMY2A/AMY2B |
| Protein name | Alpha-amylase 1/Pancreatic alpha-amylase/Alpha-amylase 2B |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 11722 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P00687 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 497039 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P00689 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from the N-terminal region of human AMY1/2. AA range:61-110 |
| Specificity | Amylase Polyclonal Antibody detects endogenous levels of Amylase protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 60kD |
| Background | Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.,cofactor:Binds 1 calcium ion per subunit.,cofactor:Binds 1 chloride ion per subunit.,online information:Amylase entry,similarity:Belongs to the glycosyl hydrolase 13 family.,subunit:Monomer., |
| Subcellular location | extracellular space,extracellular exosome, |
| Expression | Glandular pool- thyroid,Saliva,Thyroid, |
Additional Images
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Western Blot analysis of HeLa cells using Amylase Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded rat-liver, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded mouse-liver, antibody was diluted at 1:100
: AO-06-ES3894-50
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