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Antibody Product Overview
| ELK.No | ES3919 |
| Product name | Cdc34 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | CDC34; UBCH3; UBE2R1; Ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 997 |
| Human Swiss-Prot | P49427 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Cdc34 |
| Fields | >>Ubiquitin mediated proteolysis |
| Gene name | CDC34 |
| Protein name | Ubiquitin-conjugating enzyme E2 R1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 216150 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q8CFI2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human CDC34. AA range:121-170 |
| Specificity | Cdc34 Polyclonal Antibody detects endogenous levels of Cdc34 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 34kD |
| Background | The protein encoded by this gene is a member of the ubiquitin-conjugating enzyme family. Ubiquitin-conjugating enzyme catalyzes the covalent attachment of ubiquitin to other proteins. This protein is a part of the large multiprotein complex, which is required for ubiquitin-mediated degradation of cell cycle G1 regulators, and for the initiation of DNA replication. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.,function:Catalyzes the covalent attachment of ubiquitin to other proteins. May be involved in degradation of katenin.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subunit:Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. When phosphorylated, interacts with beta-TrCP (BTRC)., |
| Subcellular location | Cytoplasm. Nucleus. The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase. |
| Expression | Expressed in testes during spermatogenesis to regulate repression of cAMP-induced transcription. |
Additional Images
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Western Blot analysis of K562, PC-3, mouse liver cells using Cdc34 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-lung-cancer, antibody was diluted at 1:100
: AO-06-ES3919-100
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