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Antibody Product Overview
| ELK.No | ES3948 |
| Product name | Keap-1 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | KEAP1; INRF2; KIAA0132; KLHL19; Kelch-like ECH-associated protein 1; Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 9817 |
| Human Swiss-Prot | Q14145 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Keap1 |
| Fields | >>Ubiquitin mediated proteolysis;>>Parkinson disease;>>Pathways in cancer;>>Chemical carcinogenesis - reactive oxygen species;>>Hepatocellular carcinoma;>>Fluid shear stress and atherosclerosis |
| Gene name | KEAP1 |
| Protein name | Kelch-like ECH-associated protein 1 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 50868 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9Z2X8 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 117519 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P57790 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human KEAP1. AA range:411-460 |
| Specificity | Keap1 Polyclonal Antibody detects endogenous levels of Keap1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 70kD |
| Background | This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008], |
| Function | disease:Defects in KEAP1 may be a cause of breast cancer.,disease:Defects in KEAP1 may be involved in non small cell lung carcinomas (NSCLC) and lung adenocarcinoma.,domain:The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.,enzyme regulation:Ubiquitination and subsequent degradation of PGAM5 is inhibited by oxidative stress and sulforaphane.,function:Retains NFE2L2/NRF2 in the cytosol. Functions as substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1. Targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. May also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.,PTM:Ubiquitinated and subject to proteasomal degra |
| Subcellular location | Cytoplasm . Nucleus . Mainly cytoplasmic (PubMed:15601839). In response to selective autophagy, relocalizes to inclusion bodies following interaction with SQSTM1/p62 (PubMed:20452972). . |
| Expression | Broadly expressed, with highest levels in skeletal muscle. |
Additional Images
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Western Blot analysis of K562 cells using Keap1 Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
: AO-06-ES3948-50
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