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Antibody Product Overview
| ELK.No | ES3984 |
| Product name | CD73 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | NT5E; NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; CD73 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 4907 |
| Human Swiss-Prot | P21589 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CD73 |
| Fields | >>Purine metabolism;>>Pyrimidine metabolism;>>Nicotinate and nicotinamide metabolism;>>Metabolic pathways;>>Nucleotide metabolism |
| Gene name | NT5E |
| Protein name | 5'-nucleotidase |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q61503 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human NT5E. AA range:431-480 |
| Specificity | CD73 Polyclonal Antibody detects endogenous levels of CD73 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 68kD |
| Background | The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011], |
| Function | catalytic activity:A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate.,cofactor:Zinc.,disease:There is a decrease in the activity of NT5 in B-cell chronic lymphocytic leukemia.,function:Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.,similarity:Belongs to the 5'-nucleotidase family.,subunit:Homodimer; disulfide-linked., |
| Subcellular location | Cell membrane ; Lipid-anchor, GPI-anchor . |
| Expression | Leukocyte,Liver,Placenta,Skin, |
Additional Images
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Western Blot analysis of MCF7, L929, K562 cells using CD73 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100
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Western blot analysis of lysate from MCF7 cells, using NT5E Antibody.
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: AO-06-ES3984-50
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