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Antibody Product Overview
| ELK.No | ES4048 |
| Product name | IDH3A rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | IDH3A; Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial; Isocitric dehydrogenase subunit alpha; NAD(+)-specific ICDH subunit alpha |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 3419 |
| Human Swiss-Prot | P50213 |
| Source | Rabbit |
| Isotype | IgG |
| Target | IDH3A |
| Fields | >>Citrate cycle (TCA cycle);>>Metabolic pathways;>>Carbon metabolism;>>2-Oxocarboxylic acid metabolism;>>Biosynthesis of amino acids |
| Gene name | IDH3A |
| Protein name | Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 67834 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9D6R2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 114096 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q99NA5 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | Synthesized peptide derived from the Internal region of human IDH3A. |
| Specificity | IDH3A Polyclonal Antibody detects endogenous levels of IDH3A protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 39kD |
| Background | Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008], |
| Function | catalytic activity:Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH.,cofactor:Binds 1 magnesium or manganese ion per subunit.,similarity:Belongs to the isocitrate and isopropylmalate dehydrogenases family.,subunit:Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1., |
| Subcellular location | Mitochondrion. |
| Expression | Brain,Brain cortex,Cajal-Retzius cell,Esophagus tumor,Fetal |
Additional Images
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Western Blot analysis of MCF-7, HT29, mouse brain, mouse heart, mouse colon, mouse kidney, mouse skeletal muscle cells using IDH3A Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human heart, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-lymph-gland, antibody was diluted at 1:100
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: AO-06-ES4048-50
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