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Antibody Product Overview
| ELK.No | ES4186 |
| Product name | Rab 5A rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | RAB5A; RAB5; Ras-related protein Rab-5A |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 5868 |
| Human Swiss-Prot | P20339 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Rab 5A |
| Fields | >>Ras signaling pathway;>>Endocytosis;>>Phagosome;>>Vasopressin-regulated water reabsorption;>>Amyotrophic lateral sclerosis;>>Pathways of neurodegeneration - multiple diseases;>>Salmonella infection;>>Amoebiasis;>>Tuberculosis |
| Gene name | RAB5A |
| Protein name | Ras-related protein Rab-5A |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 271457 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9CQD1 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human RAB5A. AA range:71-120 |
| Specificity | Rab 5A Polyclonal Antibody detects endogenous levels of Rab 5A protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 24kD |
| Background | enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.,function:Required for the fusion of plasma membranes and early endosomes.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Enriched in stage I melanosomes.,subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3., |
| Function | enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP.,function:Required for the fusion of plasma membranes and early endosomes.,similarity:Belongs to the small GTPase superfamily. Rab family.,subcellular location:Enriched in stage I melanosomes.,subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3., |
| Subcellular location | Cell membrane ; Lipid-anchor ; Cytoplasmic side . Early endosome membrane ; Lipid-anchor . Melanosome . Cytoplasmic vesicle . Cell projection, ruffle . Membrane . Cytoplasm, cytosol . Cytoplasmic vesicle, phagosome membrane . Endosome membrane . Enriched in stage I melanosomes (PubMed:17081065). Alternates between membrane-bound and cytosolic forms (Probable). . |
| Expression | Brain,Cervix,Cervix carcinoma,Placenta, |
Additional Images
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Western Blot analysis of HeLa cells using Rab 5A Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
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Immunohistochemical analysis of paraffin-embedded human-lung, antibody was diluted at 1:100
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Western blot analysis of lysate from HeLa cells, using RAB5A Antibody.
: AO-06-ES4186-100
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