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Antibody Product Overview
| ELK.No | ES4251 |
| Product name | SPARC rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | SPARC; ON; SPARC; Basement-membrane protein 40; BM-40; Osteonectin; ON; Secreted protein acidic and rich in cysteine |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 6678 |
| Human Swiss-Prot | P09486 |
| Source | Rabbit |
| Isotype | IgG |
| Target | SPARC |
| Fields | |
| Gene name | SPARC |
| Protein name | SPARC |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 20692 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P07214 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 24791 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P16975 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human SPARC. AA range:141-190 |
| Specificity | SPARC Polyclonal Antibody detects endogenous levels of SPARC protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 34kD |
| Background | This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2015], |
| Function | developmental stage:Expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair.,function:Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.,online information:Osteonectin entry,similarity:Belongs to the SPARC family.,similarity:Contains 1 EF-hand domain.,similarity:Contains 1 follistatin-like domain.,similarity:Contains 1 Kazal-like domain.,subcellular location:In or around the basement membrane., |
| Subcellular location | Secreted, extracellular space, extracellular matrix, basement membrane . In or around the basement membrane. |
| Expression | Muscle,Placenta,PNS,Prostate,Skin, |
Additional Images
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Western Blot analysis of HeLa, HepG2 cells using SPARC Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded mouse-muscle, antibody was diluted at 1:100
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: AO-06-ES4251-50
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