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Antibody Product Overview
| ELK.No | ES4274 |
| Product name | Bag-3 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | BAG3; BIS; BAG family molecular chaperone regulator 3; BAG-3; Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Docking protein CAIR-1 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 9531 |
| Human Swiss-Prot | O95817 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Bag-3 |
| Fields | |
| Gene name | BAG3 |
| Protein name | BAG family molecular chaperone regulator 3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9JLV1 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human BAG3. AA range:311-360 |
| Specificity | Bag-3 Polyclonal Antibody detects endogenous levels of Bag-3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 80kD |
| Background | BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008], |
| Function | function:Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity.,similarity:Contains 1 BAG domain.,similarity:Contains 2 WW domains.,subunit:Binds to the ATPase domain of HSP70/HSC chaperones. Binds to Bcl-2 and PLC-gamma., |
| Subcellular location | Nucleus . Cytoplasm . Colocalizes with HSF1 to the nucleus upon heat stress (PubMed:26159920). . |
| Expression | Brain,Epithelium,Liver,Lung,Placenta,T-cell,Testis,Tongue, |
Additional Images
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Western Blot analysis of A549 cells using Bag-3 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100
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: AO-06-ES4274-50
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