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Antibody Product Overview
| ELK.No | ES4412 |
| Product name | CAF-1 p150 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;ELISA |
| Other name | CHAF1A; CAF; CAF1P150; Chromatin assembly factor 1 subunit A; CAF-1 subunit A; Chromatin assembly factor I p150 subunit; CAF-I 150 kDa subunit; CAF-I p150; hp150 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 10036 |
| Human Swiss-Prot | Q13111 |
| Source | Rabbit |
| Isotype | IgG |
| Target | CAF-1 p150 |
| Fields | |
| Gene name | CHAF1A |
| Protein name | Chromatin assembly factor 1 subunit A |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 27221 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9QWF0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | Synthesized peptide derived from CAF-1 p150 . at AA range: 300-380 |
| Specificity | CAF-1 p150 Polyclonal Antibody detects endogenous levels of CAF-1 p150 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 150kD |
| Background | Chromatin assembly factor I (CAF1) is a nuclear complex consisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A) subunits that assembles histone octamers onto replicating DNA in vitro (Kaufman et al., 1995 [PubMed 7600578]).[supplied by OMIM, Mar 2008], |
| Function | alternative products:Experimental confirmation may be lacking for some isoforms,developmental stage:Active complex is found in G1, S and G2 phases.,domain:Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.,function:Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenanc |
| Subcellular location | Nucleus . DNA replication foci. |
| Expression | Brain,Cervix adenocarcinoma,Epithelium,Ovary, |
Additional Images
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Western Blot analysis of K562 cells using CAF-1 p150 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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: AO-06-ES4412-100
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