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Antibody Product Overview
| ELK.No | ES4468 |
| Product name | Hec1 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IF;ELISA |
| Other name | NDC80; HEC; HEC1; KNTC2; Kinetochore protein NDC80 homolog; Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 10403 |
| Human Swiss-Prot | O14777 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Hec1 |
| Fields | |
| Gene name | NDC80 |
| Protein name | Kinetochore protein NDC80 homolog |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 67052 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9D0F1 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human KNTC2. AA range:351-400 |
| Specificity | Hec1 Polyclonal Antibody detects endogenous levels of Hec1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 73kD |
| Background | This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011], |
| Function | developmental stage:Expression peaks in mitosis.,function:Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore.,PTM:Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.,similarity:Belongs to the NDC80/HEC1 family.,subcellular location:Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore.,subunit:Component of the NDC80 complex, which consists of NDC80/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of NDC80/HEC1-CDCA1 and SPBC24-SPBC25. Each subcomplex is formed by pa |
| Subcellular location | Nucleus . Chromosome, centromere, kinetochore . Localizes to kinetochores from late prophase to anaphase (PubMed:14699129). Localizes specifically to the outer plate of the kinetochore (PubMed:14699129). . |
| Expression | Bladder,Brain,Epithelium,Lymph, |
Additional Images
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Immunofluorescence analysis of Hela cell. 1,Hec1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). Kif 7 Monoclonal Antibody(3F8)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:RS3611 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:RS3208 was diluted at 1:1000(room temperature, 50min).
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Western Blot analysis of Jurkat cells using Hec1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Immunofluorescence analysis of HUVEC cells, using KNTC2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from Jurkat cells, using KNTC2 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES4468-50
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