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Antibody Product Overview
| ELK.No | ES4521 |
| Product name | TRIM3 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | WB;IHC;IF;ELISA |
| Other name | TRIM3; BERP; RNF22; RNF97; Tripartite motif-containing protein 3; Brain-expressed RING finger protein; RING finger protein 22; RING finger protein 97 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 10612 |
| Human Swiss-Prot | O75382 |
| Source | Rabbit |
| Isotype | IgG |
| Target | TRIM3 |
| Fields | |
| Gene name | TRIM3 |
| Protein name | Tripartite motif-containing protein 3 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 55992 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9R1R2 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 83616 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | O70277 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TRIM3. AA range:1-50 |
| Specificity | TRIM3 Polyclonal Antibody detects endogenous levels of TRIM3 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 81kD |
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family, also called the 'RING-B-box-coiled-coil' (RBCC) subgroup of RING finger proteins. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to cytoplasmic filaments. It is similar to a rat protein which is a specific partner for the tail domain of myosin V, a class of myosins which are involved in the targeted transport of organelles. The rat protein can also interact with alpha-actinin-4. Thus it is suggested that this human protein may play a role in myosin V-mediated cargo transport. Alternatively spliced transcript variants encoding the same isoform have been identified. [provided by RefSeq, Jul 2008], |
| Function | domain:The interaction with myosin V is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.,similarity:Belongs to the TRIM/RBCC family.,similarity:Contains 1 B box-type zinc finger.,similarity:Contains 1 filamin repeat.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 6 NHL repeats.,subunit:Associates with myosin V and alpha-actinin-4 (ACTN4).,tissue specificity:Expressed in brain, heart, uterus and testis., |
| Subcellular location | Cytoplasm . Early endosome . Golgi apparatus, trans-Golgi network . Cell projection, dendrite . |
| Expression | Expressed in brain, heart, uterus and testis. |
Additional Images
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Western Blot analysis of COLO cells using TRIM3 Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
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Immunofluorescence analysis of NIH/3T3 cells, using TRIM3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TRIM3 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from COLO cells, using TRIM3 Antibody. The lane on the right is blocked with the synthesized peptide.
: AO-06-ES4521-100
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