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Antibody Product Overview
| ELK.No | ES4573 |
| Product name | GPR75 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IF;ELISA |
| Other name | GPR75; Probable G-protein coupled receptor 75 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 10936 |
| Human Swiss-Prot | O95800 |
| Source | Rabbit |
| Isotype | IgG |
| Target | GPR75 |
| Fields | |
| Gene name | GPR75 |
| Protein name | Probable G-protein coupled receptor 75 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 237716 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q6X632 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human GPR75. AA range:381-430 |
| Specificity | GPR75 Polyclonal Antibody detects endogenous levels of GPR75 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 59kD |
| Background | GPR75 is a member of the G protein-coupled receptor family. GPRs are cell surface receptors that activate guanine-nucleotide binding proteins upon the binding of a ligand.[supplied by OMIM, Jul 2002], |
| Function | function:Orphan receptor.,similarity:Belongs to the G-protein coupled receptor 1 family.,tissue specificity:Expressed at high levels in brain and spinal cord and at detectable levels in retinal pigment epithelium. In situ hybridization of adult eye sections localized transcripts only to the perivascular cells, surrounding retinal arterioles, in the ganglion cell/nerve fiber layer., |
| Subcellular location | Cell membrane; Multi-pass membrane protein . |
| Expression | Expressed at high levels in brain and spinal cord and at detectable levels in retinal pigment epithelium. In situ hybridization of adult eye sections localized transcripts only to the perivascular cells, surrounding retinal arterioles, in the ganglion cell/nerve fiber layer. Also expressed by islet cells (at protein level). |
Additional Images
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Western Blot analysis of HT-29 cells using GPR75 Polyclonal Antibody
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Immunofluorescence analysis of LOVO cells, using GPR75 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HT-29 and K562 cells, using GPR75 Antibody. The lane on the right is blocked with the synthesized peptide.
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: AO-06-ES4573-50
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