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Antibody Product Overview
| ELK.No | ES4574 |
| Product name | ACOT2 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;IHC;IF;ELISA |
| Other name | ACOT2; PTE2; PTE2A; Acyl-coenzyme A thioesterase 2; mitochondrial; Acyl-CoA thioesterase 2; Acyl-coenzyme A thioester hydrolase 2a; CTE-Ia; Long-chain acyl-CoA thioesterase 2; ZAP128 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 10965 |
| Human Swiss-Prot | P49753 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ACOT2 |
| Fields | >>Fatty acid elongation;>>Biosynthesis of unsaturated fatty acids;>>Metabolic pathways;>>Ovarian steroidogenesis |
| Gene name | ACOT2 |
| Protein name | Acyl-coenzyme A thioesterase 2 mitochondrial |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q9QYR9 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACOT2. AA range:171-220 |
| Specificity | ACOT2 Polyclonal Antibody detects endogenous levels of ACOT2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 53kD |
| Background | This gene encodes a member of the acyl-CoA thioesterase protein family, and is one of four acyl-CoA hydrolase genes located in a cluster on chromosome 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012], |
| Function | catalytic activity:Palmitoyl-CoA + H(2)O = CoA + palmitate.,caution:Was originally (PubMed:10944470) thought to be peroxisomal but was later shown (PubMed:16940157) to be mitochondrial.,function:Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Displays high levels of activity on medium- and long chain acyl CoAs.,similarity:Belongs to the C/M/P thioester hydrolase family.,tissue specificity:Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas., |
| Subcellular location | Mitochondrion . |
| Expression | Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas. |
Additional Images
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Western Blot analysis of VEC cells using ACOT2 Polyclonal Antibody diluted at 1:500
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western blot analysis of lysates from Jurkat and HUVEC cells, using ACOT2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HepG2 cells using ACOT2 antibody.
: AO-06-ES4574-100
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