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HSP20 (phospho Ser16) rabbit pAb
AO-06-ES4719-100
Antibody Product Overview
| ELK.No | ES4719 |
| Product name | HSP20 (phospho Ser16) rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | IHC;IF;ELISA |
| Other name | HSPB6; Heat shock protein beta-6; HspB6; Heat shock 20 kDa-like protein p20 |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 126393 |
| Human Swiss-Prot | O14558 |
| Source | Rabbit |
| Isotype | IgG |
| Target | HSP20 |
| Fields | |
| Gene name | HSPB6 |
| Protein name | Heat shock protein beta-6 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 243912 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q5EBG6 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 192245 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | P97541 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HSP20 around the phosphorylation site of Ser16. AA range:5-54 |
| Specificity | Phospho-HSP20 (S16) Polyclonal Antibody detects endogenous levels of HSP20 protein only when phosphorylated at S16. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 17kD |
| Observed band (KD) | |
| Background | This locus encodes a heat shock protein. The encoded protein likely plays a role in smooth muscle relaxation. [provided by RefSeq, Jan 2012], |
| Function | PTM:The N-terminus is blocked.,similarity:Belongs to the small heat shock protein (HSP20) family.,subunit:May exist as disulfide-linked dimer and monomer., |
| Subcellular location | Cytoplasm . Nucleus . Secreted . Translocates to nuclear foci during heat shock. . |
| Expression | Muscle,Skeletal muscle,Skin, |
Additional Images
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSP20 (Phospho-Ser16) Antibody
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Immunofluorescence analysis of HeLa cells, using HSP20 (Phospho-Ser16) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using HSP20 (Phospho-Ser16) Antibody. The picture on the right is blocked with the phospho peptide.
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