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Antibody Product Overview
| ELK.No | ES4736 |
| Product name | COL4A2 rabbit pAb |
| Reactivity | Human;Mouse |
| Applications | WB;IHC;IF;ELISA |
| Other name | COL4A2; Collagen alpha-2(IV) chain |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1284 |
| Human Swiss-Prot | P08572 |
| Source | Rabbit |
| Isotype | IgG |
| Target | Collagen IV α2 |
| Fields | >>PI3K-Akt signaling pathway;>>Focal adhesion;>>ECM-receptor interaction;>>Relaxin signaling pathway;>>AGE-RAGE signaling pathway in diabetic complications;>>Protein digestion and absorption;>>Amoebiasis;>>Human papillomavirus infection;>>Pathways in cancer;>>Small cell lung cancer |
| Gene name | COL4A2 |
| Protein name | Collagen alpha-2(IV) chain |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 12827 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | P08122 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen IV alpha2. AA range:151-200 |
| Specificity | COL4A2 Polyclonal Antibody detects endogenous levels of COL4A2 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 150kD |
| Background | This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jul 2008], |
| Function | domain:Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.,function:Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Potently inhibits angiogenesis and tumor growth.,PTM:Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.,PTM:The trimeric structure of the NC1 domains may be stabilized by covalent bonds between Lys and Met residues.,PTM:Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, lo |
| Subcellular location | Secreted, extracellular space, extracellular matrix, basement membrane. |
| Expression | Eye,Hepatocyte,Placenta,Skin, |
Additional Images
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Western Blot analysis of SH-SY5Y cells using COL4A2 Polyclonal Antibody diluted at 1:2000
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Western Blot analysis of SH-SY5Y 293T NIH-3T3 COLO cells using COL4A2 Polyclonal Antibody diluted at 1:2000
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Immunofluorescence analysis of HeLa cells, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using Collagen IV alpha2 Antibody. The picture on the right is blocked with the synthesized peptide.
: AO-06-ES4736-100
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