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Antibody Product Overview
| ELK.No | ES4753 |
| Product name | COL12A1 rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | IHC;IF;ELISA |
| Other name | COL12A1; COL12A1L; Collagen alpha-1(XII) chain |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1303 |
| Human Swiss-Prot | Q99715 |
| Source | Rabbit |
| Isotype | IgG |
| Target | COL12A1 |
| Fields | >>Protein digestion and absorption |
| Gene name | COL12A1 |
| Protein name | Collagen alpha-1(XII) chain |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | Q60847 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Collagen XII alpha1. AA range:1481-1530 |
| Specificity | COL12A1 Polyclonal Antibody detects endogenous levels of COL12A1 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 333kD |
| Observed band (KD) | |
| Background | This gene encodes the alpha chain of type XII collagen, a member of the FACIT (fibril-associated collagens with interrupted triple helices) collagen family. Type XII collagen is a homotrimer found in association with type I collagen, an association that is thought to modify the interactions between collagen I fibrils and the surrounding matrix. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008], |
| Function | alternative products:The final tissue form of collagen XII may contain homotrimers of either isoform 1 or isoform 2 or any combination of isoform 1 and isoform 2,function:Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.,PTM:Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.,PTM:O-glycosylation of isoform 1; glycosaminoglycan of chondroitin-sulfate type.,PTM:The triple-helical tail is stabilized by disulfide bonds at each end.,similarity:Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.,similarity:Contains 1 TSP N-terminal (TSPN) domain.,similarity:Contains 18 fibronectin type-III dom |
| Subcellular location | Secreted, extracellular space, extracellular matrix . |
| Expression | Found in collagen I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine, and in cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, kidney and a squamous cell carcinoma cell line. Isoform 1 is also present in the corneal epithelial Bowman's membrane (BM) and the interfibrillar matrix of the corneal stroma, but it is not detected in the limbal BM. |
Additional Images
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Immunofluorescence analysis of COS7 cells, using Collagen XII alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue, using Collagen XII alpha1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from HeLa cells using ADD2 antibody.
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: AO-06-ES4753-100
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