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Antibody Product Overview
| ELK.No | ES4761 |
| Product name | COMT rabbit pAb |
| Reactivity | Human;Rat;Mouse; |
| Applications | WB;ELISA |
| Other name | COMT; Catechol O-methyltransferase |
| Size | 100μL |
| Unit price ($) | 248 |
| Human gene ID | 1312 |
| Human Swiss-Prot | P21964 |
| Source | Rabbit |
| Isotype | IgG |
| Target | COMT |
| Fields | >>Steroid hormone biosynthesis;>>Tyrosine metabolism;>>Metabolic pathways;>>Dopaminergic synapse |
| Gene name | COMT |
| Protein name | Catechol O-methyltransferase |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | |
| Mouse gene link | |
| Mouse Swiss-Prot | O88587 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | |
| Rat gene link | |
| Rat Swiss-Prot | |
| Rat Swiss link | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human COMT. AA range:61-110 |
| Specificity | COMT Polyclonal Antibody detects endogenous levels of COMT protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | |
| Observed band (KD) | 30kD |
| Background | Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008], |
| Function | catalytic activity:S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.,cofactor:Binds 1 magnesium ion per subunit.,function:Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.,mass spectrometry: PubMed:8020475,online information:Catechol-O-methyl transferase entry,polymorphism:Low enzyme activity alleles are associated with genetic susceptibility to alcoholism [MIM:103780].,polymorphism:Two alleles, COMT*1 or COMT*H with Val-158 and COMT*2 or COMT*L with Met-158 are responsible for a three to four-fold difference in enzymatic activity.,PTM:The N-terminus is blocked.,similarity:Belongs to the mammalian catechol-O-methyltransferase family.,tissue specificity:Brain, liver, placenta, |
| Subcellular location | [Isoform Soluble]: Cytoplasm .; [Isoform Membrane-bound]: Cell membrane ; Single-pass type II membrane protein ; Extracellular side . |
| Expression | Brain, liver, placenta, lymphocytes and erythrocytes. |
Additional Images
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Western blot analysis of lysates from HUVEC and HepG2 cells, using COMT Antibody. The lane on the right is blocked with the synthesized peptide.
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Western blot analysis of the lysates from Jurkat cells using COMT antibody.
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: AO-06-ES4761-100
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