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Antibody Product Overview
| ELK.No | ES4775 |
| Product name | ACOT12 rabbit pAb |
| Reactivity | Human;Mouse;Rat |
| Applications | IHC;IF;ELISA |
| Other name | ACOT12; CACH; CACH1; STARD15; Acyl-coenzyme A thioesterase 12; Acyl-CoA thioesterase 12; Acyl-CoA thioester hydrolase 12; Cytoplasmic acetyl-CoA hydrolase 1; CACH-1; hCACH-1; START domain-containing protein 15; StARD15 |
| Size | 50μL |
| Unit price ($) | 148 |
| Human gene ID | 134526 |
| Human Swiss-Prot | Q8WYK0 |
| Source | Rabbit |
| Isotype | IgG |
| Target | ACOT12 |
| Fields | >>Pyruvate metabolism;>>Metabolic pathways |
| Gene name | ACOT12 |
| Protein name | Acyl-coenzyme A thioesterase 12 |
| Human gene link | View Human Gene |
| Human Swiss link | View Human Swiss-Prot |
| Mouse gene ID | 74156 |
| Mouse gene link | View Mouse Gene |
| Mouse Swiss-Prot | Q9DBK0 |
| Mouse Swiss link | View Mouse Swiss-Prot |
| Rat gene ID | 170570 |
| Rat gene link | View Rat Gene |
| Rat Swiss-Prot | Q99NB7 |
| Rat Swiss link | View Rat Swiss-Prot |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ACOT12. AA range:281-330 |
| Specificity | ACOT12 Polyclonal Antibody detects endogenous levels of ACOT12 protein. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Dilution | Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Storage stability | -20°C/1 year |
| Molecular Weight (Da) | 62kD |
| Observed band (KD) | |
| Background | catalytic activity:Acetyl-CoA + H(2)O = CoA + acetate.,function:Hydrolyzes acetyl-CoA to acetate and CoA.,pathway:Carbohydrate metabolism; pyruvate metabolism.,similarity:Contains 1 START domain.,similarity:Contains 2 acyl coenzyme A hydrolase domains.,subunit:Homodimer or homotetramer., |
| Function | catalytic activity:Acetyl-CoA + H(2)O = CoA + acetate.,function:Hydrolyzes acetyl-CoA to acetate and CoA.,pathway:Carbohydrate metabolism; pyruvate metabolism.,similarity:Contains 1 START domain.,similarity:Contains 2 acyl coenzyme A hydrolase domains.,subunit:Homodimer or homotetramer., |
| Subcellular location | Cytoplasm, cytosol . |
| Expression | Chondrosarcoma Lung Metastasis,Liver, |
Additional Images
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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Immunofluorescence analysis of A549 cells, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using ACOT12 Antibody. The picture on the right is blocked with the synthesized peptide.
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: AO-06-ES4775-50
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